A1
Tools
Bacteria sfGFP
What is Automation Art Interface?
It's a fluorescent bacteria artwork interface which remotely programs the Reconfigurable Automation Cart (RAC) system at Ginkgo Bioworks.
Fluorescent Bacteria Photographs
1. Publish your designed agar plate to the website gallery.
2. Approved designs are queued for automated production.
3. After incubating overnight, the artwork is photographed under UV light.
Retrieval from Storage
Travel on Magnemotion
Culture Plate into Echo 525
Agar Plate into Echo 525
Expression Strain: E. coli, BL21(DE3)
Antibiotic: Chloramphenicol at 12.5 µg/mL
Agar Plates: OmniTray or Petri Plate with LB Agar (Lennox)
Plasmid Design: Fluorescent protein genes under control of a constitutive promoter (BBa_J23106), using a strong RBS (BBa_B0034), double terminator (BBa_B0015), and 7x His tag.
Benchling example.
Sequence Optimization: Codon-optimized for E. coli and S. cerevisiae.
Photographs: Taken with an iPhone 17 and 312 nm UV lamp. Exposure, contrast, brightness, saturation, sharpness adjusted as necessary.