Opentrons Art Interface

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Note: designs are in the 'unverified' tab until approved by an admin
Gallery pictures1
Grid Standard
Bacteria ColorRed
Grid Spacing5mm
Margin2mm
Coordinates
red_points = []
green_points = []
orange_points = []

This website is made for the Opentrons recitation of 'How To Grow (Almost) Anything' (HTGAA), to teach bio-enthusiasts of all backgrounds the principles and skills at the cutting edge of bioengineering and synthetic biology.

You should write a python script that iterates over each coordinate and dispenses the correct color of bacteria into that location using the Opentrons API. Remember to switch pipette tips between each color and aspirate liquid as needed!

HTGAA 2025 Opentrons Lab Protocol

HTGAA 2025 Opentrons Lab Colab

  • Dispense 4µL points: Using 4µL per point ensures that your design prints with proportions similar to those on this website. You can experiment with different amounts, but be aware that you may get unexpected results.
  • Add an offset when necessary: To adjust for varying amounts of agar, apply a vertical offset after loading `agar_plate` in your `run()` function using the `set_offset` method:
    agar_plate.set_offset(x=0.00, y=0.00, z=17.5)
  • Use a 100mm cell culture dish: The Thermo Fisher Nunclon Delta Surface Cell Culture Dish 100 (150464) works best with MIT Lab's 3D-printed holder.
  • Take photographs: Document your process thoroughly & keep notes as you go!
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