Opentrons Art Interface

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Gallery pictures1
Grid Standard
Bacteria sfGFP
Spacing 3mm
Size1µL
Coordinates

This website is made for the Opentrons lab of 'How To Grow (Almost) Anything' (HTGAA), to teach bio-enthusiasts of all backgrounds the principles and skills at the cutting edge of bioengineering and synthetic biology.

You should write a Python script that iterates over each coordinate and dispenses the correct color of bacteria into that location using the Opentrons API. Remember to switch pipette tips between each color and aspirate liquid as needed!

HTGAA 2025 Opentrons Lab Protocol

HTGAA 2025 Opentrons Lab Colab

  • 0.5-2µL points with 3-5mm spacing as a starting point: You can experiment with different settings, but be aware that you may get unexpected results.
  • Add an offset when necessary: To adjust for varying amounts of agar, apply a vertical offset after loading `agar_plate` in your `run()` function using the `set_offset` method:
    agar_plate.set_offset(x=0.00, y=0.00, z=11.0)
  • Use a 100mm cell culture dish: The Thermo Fisher Nunclon Delta Surface Cell Culture Dish 100 (150464) works best with the MIT Lab's 3D-printed holder.
  • Take photographs: Document your process thoroughly & keep notes as you go!
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